Year: 2015 | Month: December | Volume 8 | Issue 4
A Rapid Micro-method for the Isolation of Total DNA from Barley (Hordeum vulgare L.) Tissues
DOI:10.5958/2230-732X.2015.00091.1
Abstract:
The high yielding and reproducible micro-method of barley total DNA isolation was developed. A strong buffer was used for the maximum yield of DNA. RNA was removed by treating DNA with DNase-free pancreatic RNase A and polysaccharide contamination was removed by ethanol precipitation. DNA isolated from callus or leaf tissue was proved enough for restriction enzyme digestion and Southern
blotting.
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