The present research work was carried out to evaluate the antioxidant potential and phytochemical analysis of methanol root extract of Naregamia alata. Standard methods were adopted to screen antioxidant and phytochemical nature of the plant. Antioxidant and radical scavenging activity were determined by using different in vitro assays including DPPH (2, 2 –diphenyl -1-picryl hydrazyl radical) free radical scavenging assay, Hydroxyl radical scavenging assay, Nitric oxide scavenging assay, reducing

power and superoxide free radical scavenging assay. Ascorbic acid was used as standard. The methanol root extract of the plant at concentrations of 12.5, 25, 50, 100 and 200 µg/mL were studied. The root extract of Naregamia alata showed significant antioxidant activity in a dose-dependent manner in all these assays. In Vitroantioxidant activity of the methanolic root extract showed good antioxidant power in DPPH assay (91.39%), Hydroxyl radical (76.90%) and Superoxide free radical scavenging assay (80.00%)

with IC50 values 7.5µg/ml, 45µg/ml, and 20µg/ml respectively which is almost comparable to standard antioxidant ascorbic acid. The other two assays such as Nitric oxide scavenging (55.44%) with IC50 82.5 µg/ml and Ferric reducing power (0.618) showed moderate scavenging activity. Results of phytochemical screening of the extract showed the presence of alkaloids, glycosides, terpenoids, phenols, tannin, flavanoids and coumarin.