Year: 2016 | Month: October | Volume 9 | Issue 5
In Vitro Regeneration Studies in Brassica Napus with Response to Callus Induction Frequency and Regeneration Frequency
Abstract:
The present study entitled “In vitro Regeneration Studies in Brassica napus, variety GSL- 1 and DGS-1 was carried out in the Biotechnology Laboratory, Division of Genetics and Plant Breeding SKUAST-J. Brassica napus, variety GSL 1 and DGS-1 were cultured on MS Media supplemented with different concentrations of growth regulators. The sterilization treatment with HgCl2 (0.1%) for 3 minutes resulted in highest per cent of aseptic seeds and survival of seedlings. Highest callus induction was observed in GSL-1. MS medium augmented with 2,4-D at 2-2.5 mg/l. Different concentrations of growth regulators in media showed a significant difference in the callus induction frequency (CIF) and regeneration frequency (RF) for both the genotypes tested. MS medium supplemented with BAP (5.0 mg/l) and 2, 4-D (0.5 mg/l) showed highest shoot regeneration frequency in GSL-1.
© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Print This Article Email This Article to Your Friend
