A bottle was received from a pharmaceutical company containing fragments of insect species like antennae, two leg pieces, a portion of the abdomen and two intact wing pieces at ICAR-National Bureau of Agricultural Insect Resources, Bangalore, India, for the possible identification of the insect specimen. The wing pieces given to the taxonomists suggested that the wing fragments belonged to a calyptrate dipteran - Sarcophagidae/Calliphoridae relative. Furthermore, DNA barcoding based identification was employed to determine the identity by amplifying COX1 mitochondrial gene, which was 658 bp size and GenBank accession number and barcode were generated, viz., KT368817 and VETIP006-15, respectively. Our sequence matched 100% with GenBank accession nos. GQ409351 and JF439551 and identity were determined as Pollenia rudis (Fabricius) (Diptera: Calliphoridae). The present work highlights that DNA barcoding based identification tool a powerful and imperative in determining the identity of insect, even if a part or fragment of the specimen is available. This method can be used for insect identification wherever fragments are available, which can lead to preventive measures.